3GHP

Structure of the second type II cohesin module from the adaptor ScaA scaffoldin of Acetivibrio cellulolyticus (including long C-terminal linker)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9393
Spacegroup name	P 21 21 2
Unit cell lengths	68.759, 159.221, 44.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.601 - 2.487
R-factor	0.217
Rwork	0.212
R-free	0.31700
Starting model (for MR)	3fnk
RMSD bond length	0.010
RMSD bond angle	1.481
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.487	2.487
Rmerge	0.073	0.377
Number of reflections	12802
<I/σ(I)>	20.7	2.1
Completeness [%]	71.9	24.4
Redundancy	4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10%(v/v) 2-propanol, 17%(w/v) PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K