3GHM
Crystal structure of the exosite-containing fragment of human ADAMTS13 (form-1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2007-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.667, 52.914, 76.218 |
| Unit cell angles | 90.00, 111.35, 90.00 |
Refinement procedure
| Resolution | 26.460 - 2.600 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.052 | 0.176 |
| Number of reflections | 16867 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 95.3 | 72.7 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 26% PEG1500, 100mM MES/NaOH, 8% pentaerythritol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
