3GH6
Crystal Structure of Glutathione Transferase dmgstd10 from Drosophila melanogaster, in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 73.309, 87.225, 105.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.220 - 1.650 |
| R-factor | 0.18114 |
| Rwork | 0.180 |
| R-free | 0.19669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Total number of observations | 291547 | |
| Number of reflections | 40420 | |
| <I/σ(I)> | 55.48 | 6.125 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES pH 7.5, 0.8M sodium dihydrogen phosphate, 0.8M potassium dihydrogen phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
