3GGZ
Crystal Structure of S.cerevisiae Ist1 N-terminal domain in complex with Did2 MIM motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-01 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 165.916, 165.916, 121.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.030 - 3.800 |
| R-factor | 0.289 |
| Rwork | 0.289 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 10.290 | 3.800 |
| Rmerge | 0.091 | 0.035 | 0.658 |
| Number of reflections | 17219 | ||
| <I/σ(I)> | 16.617 | ||
| Completeness [%] | 99.6 | 94.8 | 100 |
| Redundancy | 6 | 5.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Sodium Citrate, vapor diffusion, temperature 293K |
