3GGY

Crystal Structure of S.cerevisiae Ist1 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-01
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	144.845, 44.172, 66.952
Unit cell angles	90.00, 110.48, 90.00

Refinement procedure

Resolution	50.000 - 1.700
Rwork	0.226
R-free	0.24900
RMSD bond length	0.004
RMSD bond angle	0.971
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.043	0.026	0.204
Number of reflections	43548
<I/σ(I)>	30.261
Completeness [%]	98.9	98.1	95.1
Redundancy	3.7	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	PEG 3000, NaAc, MgCl2, Glycine, vapor diffusion, temperature 277K