3GGR
Crystal Structure of the Human Rad9-Hus1-Rad1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00716 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.029, 67.164, 83.405 |
Unit cell angles | 90.00, 97.58, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.200 |
R-factor | 0.291 |
Rwork | 0.289 |
R-free | 0.30600 |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.728 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.080 | 0.432 |
Number of reflections | 13010 | |
<I/σ(I)> | 4.2 | |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 8% PEG 2000 MME, 100mM Tris-HCl,200mM Trimethylamine N-oxide dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 8% PEG 2000 MME, 100mM Tris-HCl,200mM Trimethylamine N-oxide dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |