3GGQ
Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9206,0.9208 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 111.448, 111.448, 84.333 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.215 |
| Rwork | 0.198 |
| R-free | 0.24010 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | |
| Number of reflections | 24473 | |
| Completeness [%] | 92.6 | 68.2 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M HEPES, pH7.5, 12% PEG3350, 5mM cobalt chloride hexahydrate, 5mM nickel (II) chloride hexahydrate, 5mM cadmium chloride dehydrate, 5mM magnesium chloride hexahydrate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
