3GG9
CRYSTAL STRUCTURE OF putative D-3-phosphoglycerate dehydrogenase oxidoreductase from Ralstonia solanacearum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-02-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.034, 97.625, 97.808 |
| Unit cell angles | 90.00, 114.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.26138 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.098 | 0.830 |
| Number of reflections | 124032 | |
| <I/σ(I)> | 5.9 | 1 |
| Completeness [%] | 96.2 | 73.6 |
| Redundancy | 4.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100MM BIS-TRIS, 25% PEG3350, PH 5.5, 200MM AMMONIUM SULFATE, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
