3GFV
Crystal Structure of Petrobactin-binding Protein YclQ from Bacillu subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.602, 35.150, 95.433 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 41.286 - 1.750 |
| R-factor | 0.165 |
| Rwork | 0.164 |
| R-free | 0.19100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.290 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.081 | 0.489 |
| Number of reflections | 55926 | |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 4.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291 | 0.1 M Phosphate-citrate pH 4.2 40 %v/v PEG 300, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
