3GFU
FaeE-FaeG chaperone-major pilin complex of F4 ac 5/95 fimbriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.815 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.924, 90.108, 86.544 |
| Unit cell angles | 90.00, 114.49, 90.00 |
Refinement procedure
| Resolution | 28.802 - 1.991 |
| R-factor | 0.2415 |
| Rwork | 0.239 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gew |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.404 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.090 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.091 | 0.480 |
| Number of reflections | 67757 | |
| <I/σ(I)> | 9.48 | 2.6 |
| Completeness [%] | 100.0 | 78.6 |
| Redundancy | 4.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 283 | 1.6M (NH4)2SO4, 0.1M Na acetate pH 4.6 , VAPOR DIFFUSION, HANGING DROP, temperature 283K |
