3GFP
Structure of the C-terminal domain of the DEAD-box protein Dbp5
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2005-11-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795,1.0332,1.11588 |
Spacegroup name | P 62 |
Unit cell lengths | 82.423, 82.423, 56.578 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.19315 |
Rwork | 0.191 |
R-free | 0.22999 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 0.956 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.600 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.040 |
Number of reflections | 24212 |
Completeness [%] | 100.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 2M Na/K HPO4, 100mM Na cacodylate pH 6.5, 8% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |