3GFP
Structure of the C-terminal domain of the DEAD-box protein Dbp5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-11-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795,1.0332,1.11588 |
| Spacegroup name | P 62 |
| Unit cell lengths | 82.423, 82.423, 56.578 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.19315 |
| Rwork | 0.191 |
| R-free | 0.22999 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.956 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.600 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.040 |
| Number of reflections | 24212 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 2M Na/K HPO4, 100mM Na cacodylate pH 6.5, 8% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
