3GFL

Crystal structure of the ST1710 mutant (R90A) protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	180
Detector technology	IMAGE PLATE
Collection date	2008-09-26
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54178
Spacegroup name	P 41 21 2
Unit cell lengths	45.905, 45.905, 137.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.670 - 1.900
R-factor	0.205
Rwork	0.205
R-free	0.23900
Starting model (for MR)	2eb7
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	12085
Completeness [%]	97.7	95.1
Redundancy	13	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18% PEG 8000, 0.2M Calcium acetate, 0.1M Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K