3GFG
Structure of putative oxidoreductase yvaA from Bacillus subtilis in triclinic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 72.931, 92.135, 187.589 |
Unit cell angles | 95.31, 94.19, 95.21 |
Refinement procedure
Resolution | 45.410 - 2.590 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gdo |
RMSD bond length | 0.012 |
RMSD bond angle | 1.335 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.084 | 0.349 |
Number of reflections | 146417 | |
<I/σ(I)> | 11.381 | 2.77 |
Completeness [%] | 98.3 | 97.9 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M Bis-tris pH 6.5, 25% PEG 3350, 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |