3GF7

Glutaconyl-coA decarboxylase A subunit from Clostridium symbiosum apoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-05
Detector	MAR CCD 165 mm
Wavelength(s)	0.80150
Spacegroup name	F 2 2 2
Unit cell lengths	102.050, 143.530, 167.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.480 - 2.400
R-factor	0.187
Rwork	0.185
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pix
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.987	34.220	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge	0.074	0.038	0.334
Total number of observations		4569	21545
Number of reflections	24154
<I/σ(I)>	9.07	12.9	2.3
Completeness [%]	99.9	98.2	100
Redundancy	6.1	5.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1