3GEW
FaeE-FaeG chaperone-major pilin complex of F4 ad fimbriae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.576, 89.757, 85.826 |
Unit cell angles | 90.00, 113.94, 90.00 |
Refinement procedure
Resolution | 17.890 - 2.000 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3F65 AND 2J6G |
RMSD bond length | 0.008 |
RMSD bond angle | 1.157 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.090 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.105 | 0.530 |
Number of reflections | 68837 | |
<I/σ(I)> | 10.91 | 2.98 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 283 | 2M (NH4)2SO4, 0.1M Na acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 283K |