3GEU
Crystal Structure of IcaR from Staphylococcus aureus, a member of the tetracycline repressor protein family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-12-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97624 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 113.922, 121.141, 61.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.420 - 1.900 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22800 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.501 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.474 |
Number of reflections | 67319 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 99.3 | 93.7 |
Redundancy | 7.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | PEG3350 20%, 0.2M NH4 Formate, pH 7, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 7 | 298 | PEG3350 20%, 0.2M NH4 Formate, pH 7, VAPOR DIFFUSION, temperature 298K |
1 | VAPOR DIFFUSION | 7 | 298 | PEG3350 20%, 0.2M NH4 Formate, pH 7, VAPOR DIFFUSION, temperature 298K |