3GET

Crystal structure of putative histidinol-phosphate aminotransferase (NP_281508.1) from Campylobacter jejuni at 2.01 A resolution

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	53.745, 79.420, 166.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.549 - 2.010
R-factor	0.177
Rwork	0.174
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	1.676
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.549	45.549	2.080
High resolution limit [Å]	2.010	4.320	2.010
Rmerge	0.085	0.040	0.525
Number of reflections	46287	5153	3549
<I/σ(I)>		19.6	2
Completeness [%]	95.3	99	75.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	NANODROP, 10.0% Isopropanol, 20.0% PEG 4000, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K