3GEO
SULFITE REDUCTASE HEMOPROTEIN NITRITE COMPLEX
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03-09 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.800, 77.400, 87.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.185 |
Rwork | 0.185 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aop |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.078 * | 0.291 * |
Number of reflections | 28149 | |
<I/σ(I)> | 16.4 | 4.2 |
Completeness [%] | 98.9 | 98.6 * |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.7 | pH 7.7 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | reservoir | potassium phosphate | 65 (mM) | |
3 | 1 | reservoir | EDTA | 0.1 (M) | |
4 | 1 | reservoir | PEGMW8000 | 15 (%) |