3GEI
Crystal structure of MnmE from Chlorobium tepidum in complex with GCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 139.882, 224.572, 156.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 3.400 |
| R-factor | 0.24624 |
| Rwork | 0.245 |
| R-free | 0.26891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xzp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.425 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.129 | 0.654 |
| Number of reflections | 34042 | |
| <I/σ(I)> | 12.13 | 2.02 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 7.45 | 7.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 46mM NaOH, 12% PEG 4000, 40mM NaCl, 5mM GCP, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
