3GEH
Crystal structure of MnmE from Nostoc in complex with GDP, FOLINIC ACID and ZN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.28186 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.279, 124.279, 174.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 3.200 |
R-factor | 0.24192 |
Rwork | 0.241 |
R-free | 0.26743 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xzp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.185 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.127 | 0.485 |
Number of reflections | 23100 | |
<I/σ(I)> | 8.61 | 2.18 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 7.8 | 8.03 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM TRIS-HCl, pH 7.5, 22% (w/v) PEG 550 MME, 10mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |