3GEE
Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98003 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 130.804, 130.804, 200.611 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.950 |
R-factor | 0.23383 |
Rwork | 0.232 |
R-free | 0.26951 |
Structure solution method | MIR |
Starting model (for MR) | 1xzp |
RMSD bond length | 0.006 |
RMSD bond angle | 1.157 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.077 | 0.717 |
Number of reflections | 18513 | |
<I/σ(I)> | 24.5 | 2.4 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 7.16 | 7.28 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100mM TRIS-HCl, pH 8.5, 2.25M NaCl, 15% (w/v) PEG 6000, 5mM GDP, 5mM AlCl3, 50mM NaF, VAPOR DIFFUSION, HANGING DROP, temperature 277K |