3GEE
Crystal structure of MnmE from Chlorobium tepidum in complex with GDP and FOLINIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98003 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 130.804, 130.804, 200.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.950 |
| R-factor | 0.23383 |
| Rwork | 0.232 |
| R-free | 0.26951 |
| Structure solution method | MIR |
| Starting model (for MR) | 1xzp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.157 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.077 | 0.717 |
| Number of reflections | 18513 | |
| <I/σ(I)> | 24.5 | 2.4 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 7.16 | 7.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100mM TRIS-HCl, pH 8.5, 2.25M NaCl, 15% (w/v) PEG 6000, 5mM GDP, 5mM AlCl3, 50mM NaF, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
