3GE9
A Structurally Atypical ThyX from Corynebacterium glutamicum NCHU 87078 Is Not Required for Thymidylate Biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 77 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 42 2 2 |
Unit cell lengths | 96.322, 96.322, 64.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.500 - 2.610 |
R-factor | 0.23439 |
Rwork | 0.232 |
R-free | 0.28411 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.689 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.388 |
Number of reflections | 9629 | |
<I/σ(I)> | 28.7 | |
Completeness [%] | 99.0 | 99.2 |
Redundancy | 9.1 | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |