3GE4

Crystal structure of ferritin:DNA-binding protein DPS from Brucella Melitensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-06
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	95.240, 98.330, 105.450
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	19.860 - 1.700
R-factor	0.145
Rwork	0.143
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9r dodecamer modified with ccp4 program chainsaw
RMSD bond length	0.016
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.089	0.609
Number of reflections	212707
<I/σ(I)>	13.35	2.1
Completeness [%]	99.8	99.9
Redundancy	3.73	3.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	PACT SCREEN, E2: 20% PEG 3350, 200MM NABR, BRABA.00136.A AT 24.4MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K