3GDZ
Crystal structure of arginyl-tRNA synthetase from Klebsiella pneumoniae subsp. pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-06 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.351, 41.966, 71.464 |
| Unit cell angles | 90.00, 91.11, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.19516 |
| Rwork | 0.192 |
| R-free | 0.24777 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.153 | 0.557 |
| Number of reflections | 21569 | |
| <I/σ(I)> | 17.5 | 3.11 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 8.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES pH 7.5, 1.4M Sodium citrate tribasic dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
