3GDP
Hydroxynitrile lyase from almond, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8439 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.050, 93.710, 87.260 |
| Unit cell angles | 90.00, 106.39, 90.00 |
Refinement procedure
| Resolution | 38.250 - 1.570 |
| Rwork | 0.186 |
| R-free | 0.21000 |
| Starting model (for MR) | 1ju2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.670 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 143518 | |
| <I/σ(I)> | 11.2 | 2.6 |
| Completeness [%] | 96.7 | 89.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 20% w/v PEG-4000, 16% v/v isopropanol, pH 7.0, VAPOR DIFFUSION, temperature 298K |
