3GDJ
Crystal structure determination of camel(Camelus dromedarius)hemoglobin at 2 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-19 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5417 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.759, 116.782, 52.807 |
Unit cell angles | 90.00, 120.07, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.23062 |
Rwork | 0.224 |
R-free | 0.29471 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pgh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.118 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.077 | 0.320 |
Number of reflections | 35552 | |
<I/σ(I)> | 5.2 | 1 |
Completeness [%] | 94.2 | 92.4 |
Redundancy | 2.43 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.003ml of protein solution with 0.002ml of 45% PEG 3350 in 50mM of phosphate buffer at pH 6.8 with 1 M NaCl equilibrated against 1 ml of reservoir containing the same solution, VAPOR DIFFUSION, HANGING DROP, temperature 293KK |