3GDC
Crystal structure of multicopper oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.38 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.956, 50.705, 134.667 |
| Unit cell angles | 90.00, 107.86, 90.00 |
Refinement procedure
| Resolution | 128.000 - 1.800 |
| R-factor | 0.19283 |
| Rwork | 0.191 |
| R-free | 0.22471 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.182 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 128.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 75847 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 0.1 M imidazole, 0.2 M CaOAc, 0.1 M CaCl2, 12% polyethyleneglycol 8000, pH 8.0, VAPOR DIFFUSION, temperature 298K |
