3GD0
Crystal structure of laminaripentaose-producing beta-1,3-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2006-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.164, 60.683, 149.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.620 |
| R-factor | 0.18135 |
| Rwork | 0.179 |
| R-free | 0.21768 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.060 | 0.336 |
| Number of reflections | 54285 | |
| <I/σ(I)> | 43.9 | 8.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 293 | 0.15M ammonium sulfate, 30% PEG MME5000, 0.1M MES (pH6.5), LIQUID DIFFUSION, temperature 293K |
| 2 |
