3GCV
Human P38 MAP Kinase in Complex with RL62
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54170 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.785, 70.003, 74.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.880 - 2.300 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zyj |
RMSD bond length | 0.013 |
RMSD bond angle | 1.472 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.256 |
Number of reflections | 16273 | 1930 |
<I/σ(I)> | 17.06 | 6.3 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 4.63 | 4.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |