3GCT

STRUCTURE OF GAMMA-*CHYMOTRYPSIN IN THE RANGE $P*H 2.0 TO $P*H 10.5 SUGGESTS THAT GAMMA-CHYMOTRYPSIN IS A COVALENT ACYL-ENZYME ADDUCT AT LOW $P*H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	285
Detector technology	FILM
Detector	KODAK
Spacegroup name	P 42 21 2
Unit cell lengths	70.000, 70.000, 97.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.600
Rwork	0.173
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019 *
RMSD bond angle	17.500 *
Data reduction software	OSCTST
Data scaling software	AGROVATA/ROTAVATE
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.600 *
Rmerge	0.066 *
Total number of observations	58476 *
Number of reflections	23255 *
Completeness [%]	70.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7 *		pH 10.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	50 (%sat)