3GCB
GAL6 (YEAST BLEOMYCIN HYDROLASE) MUTANT C73A/DELTAK454
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 150.547, 150.547, 90.134 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.870 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 21.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.042 | 0.062 |
| Total number of observations | 199974 * | |
| Number of reflections | 49450 | |
| <I/σ(I)> | 39 | 22 |
| Completeness [%] | 98.8 | 96.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE AND PEG MME 2K., pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | bleomycin | 0.5 (mM) | |
| 3 | 1 | drop | Tris-HCl | 25 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 5 | 1 | reservoir | mPEG2000 | 3 (%(v/v)) |
