3GC3
Crystal Structure of Arrestin2S and Clathrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-01 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.857, 126.173, 129.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.070 - 2.200 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.25100 |
Structure solution method | MIR |
Starting model (for MR) | 1g4m |
RMSD bond length | 0.031 |
RMSD bond angle | 2.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0034) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.056 |
Number of reflections | 61963 |
<I/σ(I)> | 21 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM bis_tris propane 4 -4.5 M ammonium acetate 4.5% ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |