3GBZ
Structure of the CMGC CDK Kinase from Giardia lamblia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-14 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.720, 73.050, 75.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.040 - 1.850 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | MR, MR |
| Starting model (for MR) | 1oit modified by ccp4 program chainsaw |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.045 | 0.498 |
| Number of reflections | 25330 | |
| <I/σ(I)> | 19.58 | 2.1 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 20% PEG 3350, 200MM K/NA TARTRATE, 0.4UL + 0.4UL PROTEIN AT 27 MG/ML, PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
