3GBX
Serine hydroxymethyltransferase from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.955, 49.734, 94.736 |
| Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
| Resolution | 45.300 - 1.800 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.19100 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.662 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | 0.779 |
| Number of reflections | 60173 | |
| <I/σ(I)> | 21.273 | 2.39 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M sodium acetate, 0.1 M MES buffer, 30% PEG-2000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
