3GBL

Crystal structure of grass carp Beta2-microglobulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.740, 40.601, 71.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.19809
Rwork	0.195
R-free	0.25206
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lds
RMSD bond length	0.012
RMSD bond angle	1.389
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.560
High resolution limit [Å]	2.000
Number of reflections	6986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	291	0.1M MES pH 6.5, 12% PEG 20000, 3%(v/v) ethanol, EVAPORATION, temperature 291K