3GB7

Potassium Channel KcsA-Fab complex in Li+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	157.064, 157.064, 75.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.660 - 2.850
R-factor	0.239
Rwork	0.239
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4d
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.850
Number of reflections	20652
<I/σ(I)>	25.2
Completeness [%]	95.5
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	24% PEG 400, 100 mM MES, 50 mM Magnesium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K