3GAX
Crystal structure of monomeric human cystatin C stabilized against aggregation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 |
Unit cell lengths | 76.260, 76.260, 97.576 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.260 - 1.700 |
R-factor | 0.13926 |
Rwork | 0.138 |
R-free | 0.16720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g96 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.896 |
Number of reflections | 35237 | |
<I/σ(I)> | 25.9 | 2 |
Completeness [%] | 99.8 | 98.3 |
Redundancy | 10.5 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 1.25M Ammonium phosphate, 150mM Sodium phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |