3GAQ
Female-specific Histamine-Binding Protein, D24R Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.963, 56.610, 63.801 |
| Unit cell angles | 90.00, 107.08, 90.00 |
Refinement procedure
| Resolution | 11.960 - 2.250 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qft |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.279 |
| Data reduction software | MOSFLM |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.300 |
| Number of reflections | 18800 | |
| <I/σ(I)> | 15.7 | 4.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | Protein at 10 mg/ml mixed in a 1:1 ratio with reservoir containing: 2.6 M Ammonium Sulphate, 0.1 M Bicine, pH 8.2, and 10 % isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
