3GAO
Crystal structure of the guanine riboswitch bound to xanthine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-11-29 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.351, 35.099, 42.248 |
Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
Resolution | 19.490 - 1.900 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1U8D |
RMSD bond length | 0.004 |
RMSD bond angle | 1.100 |
Data reduction software | d*TREK |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.500 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 14909 | |
<I/σ(I)> | 10.9 | 4.4 |
Completeness [%] | 96.4 | |
Redundancy | 3.13 | 2.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 200 mM ammonium acetate, 20 % PEG 2K, 12 mM cobalt hexammine, 10 mM K+ HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Ammonium acetate | ||
2 | 1 | 1 | PEG2000 | ||
3 | 1 | 1 | Cobalt hexammine | ||
4 | 1 | 1 | K+ HEPES | ||
5 | 1 | 2 | Ammonium acetate | ||
6 | 1 | 2 | PEG2000 | ||
7 | 1 | 2 | Cobalt hexammine | ||
8 | 1 | 2 | K+ HEPES |