3GAB
C-terminal domain of Bacillus subtilis MutL crystal form I
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792, 0.9794, 0.9686 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.288, 94.894, 218.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.778 - 2.500 |
R-factor | 0.2189 |
Rwork | 0.216 |
R-free | 0.26700 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.609 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.040 | 0.225 |
Number of reflections | 35660 | |
<I/σ(I)> | 41.5 | 4.5 |
Completeness [%] | 95.9 | 76 |
Redundancy | 5.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 24-29% PEG MME 550, 0.1 M MgCl2, 0.1 M TRIS pH 9, 5% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 24-29% PEG MME 550, 0.1 M MgCl2, 0.1 M TRIS pH 9, 5% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |