3GA5
X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with (2R)-glyceryl-beta-D-galactopyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-12 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.955 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.458, 109.275, 150.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.740 - 1.870 |
| R-factor | 0.17273 |
| Rwork | 0.170 |
| R-free | 0.22219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gca |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.130 | 0.435 |
| Number of reflections | 49021 | |
| <I/σ(I)> | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 20%(w/v) polyethylene, glycol 3350, 0.2M NaSC, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
