3GA4
Crystal structure of Ost6L (photoreduced form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.85506 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.989, 61.076, 106.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.300 |
R-factor | 0.1548 |
Rwork | 0.153 |
R-free | 0.18730 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Ost6L structure solved by SAD (bromide signal) |
RMSD bond length | 0.009 |
RMSD bond angle | 1.266 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.400 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 47810 | |
<I/σ(I)> | 22.4 | 4.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 7.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% PEG 3350, 50 mM Na-citrate, pH 3.0, protein solution buffer: 50 mM Tris-HCl, pH 8.0 , VAPOR DIFFUSION, SITTING DROP, temperature 277K |