3GA2

Crystal structure of the Endonuclease_V (BSU36170) from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR624

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97902
Spacegroup name	P 21 21 2
Unit cell lengths	61.806, 83.861, 52.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.100
R-factor	0.2
Rwork	0.200
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SnB (then SOLVE/RESOLVE)
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.095	0.353
Number of reflections	30177
<I/σ(I)>	20.1	3.8
Completeness [%]	98.2	88.2
Redundancy	7.2	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 16% PEG 3350 and 200 mM ammonium tartrate., VAPOR DIFFUSION, HANGING DROP, temperature 291K