3G9V
Crystal structure of a soluble decoy receptor IL-22BP bound to interleukin-22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 41 |
| Unit cell lengths | 67.948, 67.948, 172.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.842 - 2.756 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dlq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.233 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 19525 | |
| <I/σ(I)> | 1.61 | 1.61 |
| Completeness [%] | 96.6 | 98 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 291 | 25mM Bicine, 150mM NaCl, 1mM CHAPS, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
