3G9O
GR DNA-binding domain:Sgk 16bp complex-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.382, 38.470, 97.039 |
| Unit cell angles | 90.00, 123.44, 90.00 |
Refinement procedure
| Resolution | 34.354 - 1.650 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.678 |
| Number of reflections | 39980 | |
| <I/σ(I)> | 16.4 | 1.5 |
| Completeness [%] | 91.4 | 51.2 |
| Redundancy | 6.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM MES, pH 6.0, 100mM KCl, 10mM MgCl2, 10% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | MES | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | PEG400 |
