3G99
GR DNA binding domain:Pal complex-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115894 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.774, 38.189, 97.064 |
| Unit cell angles | 90.00, 123.11, 90.00 |
Refinement procedure
| Resolution | 35.574 - 1.810 |
| R-factor | 0.1941 |
| Rwork | 0.191 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1glu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.068 | 0.633 |
| Number of reflections | 24846 | |
| <I/σ(I)> | 14.9 | 1.1 |
| Completeness [%] | 73.8 | 10 |
| Redundancy | 4.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM MES, pH 6.0, 100 KCl, 10mM MgCl2, 10% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | MES | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | PEG 400 |
