3G8R
Crystal structure of putative spore coat polysaccharide biosynthesis protein E from Chromobacterium violaceum ATCC 12472
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 107.672, 107.672, 164.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.490 |
R-factor | 0.212 |
Rwork | 0.208 |
R-free | 0.27800 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.769 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.520 |
High resolution limit [Å] | 2.480 | 6.730 | 2.480 |
Rmerge | 0.143 | 0.052 | 0.699 |
Number of reflections | 57128 | ||
<I/σ(I)> | 13.212 | ||
Completeness [%] | 88.0 | 98.6 | 35.6 |
Redundancy | 5.2 | 5.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.1 M DL-maleic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |