3G8H
Crystal structure of phospholipase A2 ammodytoxin C from vipera ammodytes ammodytes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-01-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97565 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 67.730, 67.730, 45.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.330 - 1.350 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zwp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.084 | 0.661 |
| Number of reflections | 25781 | |
| <I/σ(I)> | 6.7 | 1.2 |
| Completeness [%] | 96.5 | 79.8 |
| Redundancy | 10 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 1.2M (NH4)2SO4, 0.1M IMIDAZOLE, 0.2M (LI)2SO4, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
