3G8G
Crystal structure of phospholipase A2 ammodytoxin A from vipera ammodytes ammodytes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-03-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 68.190, 68.190, 46.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.527 - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zwp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.527 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.143 | 0.791 |
| Number of reflections | 13933 | |
| <I/σ(I)> | 4.7 | 1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.4 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 1.26M (NH4)2SO4, 0.1M TRIS, 0.2M (LI)2SO4, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
