3G88
T. thermophilus 16S rRNA G527 methyltransferase in complex with AdoMet in space group P61
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-16 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 61 |
Unit cell lengths | 77.847, 77.847, 167.665 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.380 - 1.870 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.20000 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.483 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.089 | 0.485 |
Number of reflections | 47411 | |
<I/σ(I)> | 2.4 | |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 4.5 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | 20% (w/v) PEG1000, 100 mM imidazole (pH 8.0) and 200 mM calcium acetate, microbatch technique under oil, temperature 277K |